• This topic has 4 replies, 2 voices, and was last updated 3 weeks ago by Sabine SuppmannSabine Suppmann.
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    • November 14, 2019 at 14:16 #2586
      Sabine Suppmann
      Sabine Suppmann
      Moderator

      Dear All
      has anyone ever tried the combination VEGF-N-Strep tag resulting in secretion + proper processing of the signal sequence?
      Best
      Sabine

    • April 23, 2021 at 16:04 #3729
      Nick Berrow
      Nick Berrow
      Moderator

      Hi Sabine,
      Sorry I only just saw this post…
      We inserted a OneStrep-3C between a signal peptide and a receptor ECD and saw no reduction in secreted protein compared with the C-his version.
      We used:
      MGILPSPGMPALLSLVSLLSVLLMGCVA¡ETGASWSHPQFEKGGGSGGGSGGGSSWSHPQFEKSSGLEVLFQGP…

      Which is µphosphatase leader-OneStrep-3C. This worked well for us but we’ve never tried it with VEGF.
      Best
      Nick

    • April 23, 2021 at 22:11 #3732
      Sabine Suppmann
      Sabine Suppmann
      Moderator

      Hi Nick
      better late than never! Tanks, in the meantime I learned that VEGF SS and TwinStrep can work
      best
      Sabine

    • April 23, 2021 at 22:11 #3733
      Sabine Suppmann
      Sabine Suppmann
      Moderator

      Hi Nick
      better late than never! Tanks, in the meantime I learned that VEGF SS and TwinStrep can work
      best
      Sabine

    • April 23, 2021 at 22:11 #3734
      Sabine Suppmann
      Sabine Suppmann
      Moderator

      Hi Nick
      better late than never! Tanks, in the meantime I learned that VEGF SS and TwinStrep can work
      best
      Sabine

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