• This topic has 2 replies, 3 voices, and was last updated 7 months ago by Joop van den Heuvel.
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    • October 6, 2024 at 16:27 #5468

      Yael Nir Keren
      Participant

      Hi everyone,

      Could you recommend a reliable source for H5/T.ni cells? We are looking to purchase some.

      Thank you!

      Yael

    • October 14, 2024 at 15:27 #5470

      Regis Lemaitre
      Participant

      Dear Yael,

      we are using T.ni from Expression Systems (cat# 94-002F),
      with ESF921 medium from Expression Systems (cat# 94-00101).

      We are satisfied with them.
      We grow them at @27ºC, but we use them also at @22ºC for protein production.
      It is protein dependant, we advise to try what works best for you.

      cheers

      Regis

    • October 14, 2024 at 16:23 #5471

      Joop van den Heuvel
      Participant

      Daer Yael,
      the High Five of Thermo Fisher (BTI-TN-5B1-4) is also very good. We have adapted these cells to EXCELL405 medium from Sigma. This is the best for TGE with our pOpIE2 expression vectors. The Express Five medium is also a good alternative. Use high passage cells even for that medium as wel. We have good results at 27 °C.

      Best regards Joop

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