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    • November 5, 2019 at 10:03 #2569

      Jan Blaha
      Participant

      Hello P4EU,

      I am wondering if anyone tried mitigating cell division of insect cells for transient gene expression? Do you think it could boost expression levels of transiently transfected Hi5 cultures? Does anyone have experience with valproic/butyric acid effect on Hi5 cells? Do you think infection with “empty” baculovirus after transient transfection could help boosting expression of GOI on TGE vector by arresting cell division due to the infection?

      Cheers,

      Jan

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