Viewing 1 reply thread
  • Author
    Posts
    • June 18, 2024 at 09:10 #5221

      Kelvin Lau
      Participant

      For those of you working with multi-subunit systems that are also in need of testing combinations, tags and hosts, a user at our facility developed this hybrid Golden-Gate / Gibson cloning system.

      They have adapted it for bacterial, insect and mammalian expression systems

      They are always open for suggestions (expanding the vector base, promoters, tags etc), so please let them know your comments!

      https://www.biorxiv.org/content/10.1101/2024.06.17.599261v1

    • June 21, 2024 at 11:01 #5222

      Martine Vanhoucke
      Participant

      Dear Kelvin

      It would be interesting to make this system publicly available via a culture collection.
      I noticed in the preprint that you will deposit the vectors in Addgene. Is there any chance to still (or also) deposit them in the BCCM/GeneCorner Plasmid collection, which is also a P4EU partner? For sure, this will make it more easily accessible for European scientists.
      You will find a GeneCorner poster at the 20th P4EU meeting.
      If you are interested, please contact me via e-mail: bccm.genecorner@ugent.be.

  • Author
    Posts
Viewing 1 reply thread

You must be logged in to reply to this topic.