Scope
The objective of this course is to familiarize participants with these guidelines for protein quality control and to provide an overview of the technologies essential for enhancing the accuracy and reproducibility of data obtained with purified proteins. Throughout the course, participants will have access to the theoretical foundations of these technologies and will also gain hands-on experience with them, ensuring a comprehensive learning experience.

Target audience
This course is designed for scientists currently using or intending to use purified proteins in their laboratory experiments. By the end of the course, participants should be familiar with a variety of techniques applicable to purified proteins, ultimately enhancing the reliability and reproducibility of data in downstream applications involving these proteins.

Programme
We offer a 4-day course comprising lectures on protein expression and purification techniques, and methods for assessing the quality of purified protein samples. Additionally, participants will have hands-on practical sessions demonstrating how to apply these experiments for protein quality control. Topics included in the course:

Lectures

Day 1
Protein expression, purification and QC; Overview of protein expression methods; Overview of protein purification methods; Guidelines for protein quality control

Day 2
PAGE, Protein concentration and optical properties of protein; Determination of protein concentration; Polyacrylamide gel electrophoresis;
Absorbance, fluorescence, circular dichroism; Hydrogen-deuterium exchange applications in protein quality control

Day 3
Quality control tests; Light scattering and mass photometry; Mass spectrometry;

Day 4
Storage, formulation and extended tests; Differential scanning fluorimetry; Protein storage and formulation; Protein-protein interactions

Practical sessions

Session 1 : DLS, nanoDSF, SEC-MALS
Session 2 : Mass spectrometry, mass photometry
Session 3 : UV absorbance, fluorescence, circular dichroism

For further information and registration please visit:
https://www.congressi.unisi.it/icPQC-Siena2025/

Please note that the closing date for applications is April 13th, 2025