Freezing Baculovirus-infected insect cells (BIIC) stocks
Authors Name:
Imre Berger and Fruszina Rabi
Protocol Type:
Author Protocol
Authors Email
imre.berger@bristol.ac.uk
Affiliation
University of Bristol, School of Biochemistry
Authors list:
Imre Berger and Fruszina Rabi
Background:
Cryo-preservation of recombinant baculoviruses within the insect host cells is an efficient method of long-term baculovirus storage. This method from the Berger lab is based on the original paper:
The titerless infected-cells preservation and scale-up (TIPS) method for large-scale production of NO-sensitive human soluble guanylate cyclase (sGC) from insect cells infected with recombinant baculovirus
David J Wasilko 1 , S Edward Lee, Kim J Stutzman-Engwall, Beverly A Reitz, Thomas L Emmons, Karl J Mathis, Michael J Bienkowski, Alfredo G Tomasselli, H David Fischer.
Protein Expr Purif. 2009 Jun;65(2):122-32. doi: 10.1016/j.pep.2009.01.002. Epub 2009 Jan 11.
Comments
No comment yet.