Freezing Baculovirus-infected insect cells (BIIC) stocks

Authors Name:
Imre Berger and Fruszina Rabi

Protocol Type:
Author Protocol

University of Bristol, School of Biochemistry

Authors list:
Imre Berger and Fruszina Rabi


Cryo-preservation of recombinant baculoviruses within the insect host cells is an efficient method of long-term baculovirus storage. This method from the Berger lab is based on the original paper:
The titerless infected-cells preservation and scale-up (TIPS) method for large-scale production of NO-sensitive human soluble guanylate cyclase (sGC) from insect cells infected with recombinant baculovirus
David J Wasilko 1 , S Edward Lee, Kim J Stutzman-Engwall, Beverly A Reitz, Thomas L Emmons, Karl J Mathis, Michael J Bienkowski, Alfredo G Tomasselli, H David Fischer.
Protein Expr Purif. 2009 Jun;65(2):122-32. doi: 10.1016/j.pep.2009.01.002. Epub 2009 Jan 11.


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