We are pleased to present the 1st P4EU Structural Biology Workshop Series 2023. The series was composed of four interactive on-line sessions of approximately two hours.

Programme at a glance

3.11.2023       Alphafold and complementary topics
17.11.2023    Coot map inspection and PyMol protein visualization
1.12.2023      ChimeraX
15.12.2023    Experience-based construct design

3.11.2023 Kelvin Lau

Alphafold and complementary topics

In this session, we will introduce freely available tools (Alphafold2, UNIPROT, predictors of transmembrane segments and signal peptides etc) to help in guiding your construct designs. We will introduce these tools and how to use them individually and as part of a workflow that one could implement or modify to their own needs.

You can download the session video capture (179MB mp4 file) here:
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17.11.2023 Luigi Scietti and Anja Schütz

Coot map inspection and PyMol protein visualization

In this interactive session, participants will learn what’s a PDB file and how to look for a protein structure in the Protein Data Bank (PDB). They will see how to open and inspect electron density maps using Coot software. In the second part, participants will interactively follow a tutorial to learn how to open, inspect and represent protein structure using PyMol.

You can download the session video capture (204MB mp4 file) here:
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The instructions for Luigi’s session can be downloaded here (pdf):
PyMol tutorial-Luigi Scietti

1.12.2023 Aljaz Gaber

ChimeraX

In this interactive session, participants will learn how to import, visualize, and analyze proteins and protein complexes using the ChimeraX software. From the basics of protein structure to advanced techniques for visualizing protein-ligand interactions and protein-protein interfaces, this workshop will equip attendees with valuable skills for structural biology research.

The instructions for Aljaz’s session can be downloaded here (pdf):
ChimeraX tutorial-Aljaz Gaber

You can download the session video capture (201MB mp4 file) here:
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15.12.2023 Anja Schütz, Kelvin Lau, Luigi Scietti and Aljaz Gaber, Kim Remans

Experience-based construct design

In this session we held a virtual round table discussion with structural biology experts about their experiences, preferences and the do’s and dont’s of construct design.

The basis for our round table discussion was a list of questions collected in advance from participants:
Round Table Questions 15122023

You can download the session video capture (129MB mp4 file) here:
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The chat log is also available here:
Round Table Chat 15122023

Thanks to Sabine Suppmann (Fraunhofer ITMP) for organizing this series, Kim and Kelvin for organizing the on-line sessions and recordings and all of the speakers/tutors for their contributions.