P4EU P4EU P4EU
Navigation
  • Home
  • About Us
    • Executive Board Members
    • Network Members
    • Members Publications
  • News
    • Projects & Benchmarks
    • Courses
    • Upcoming P4EU Meetings
    • Upcoming Conferences
  • Join Us
  • Protein Quality Standard PQS
  • Jobs
  • Contact
  • Archives
    • Courses
    • P4EU Meetings

Login:

Lost Password?

Shortcuts

  • Public Forum
  • Wiki
  • Contact

Protein Quality Standard PQS

There is increasing awareness in the scientific community about the lack of reproducibility and reliability of results published with purified proteins. While protein production is highly regulated and controlled in the pharmaceutical industry by the authorities, there are up to date no guidelines or standards in place in the academic research to guarantee the quality of proteins included in scientific experiments. There is an urgent need to define guidelines for the scientists but also for editors and reviewers of scientific journals and funding agencies for their review processes:

Lebendiker M., Danieli T. and de Marco A. (2014)
Raynal B., Lenormand P., Baron B., Hoos S. and England P.(2014)
Berrow N., de Marco A., Lebendiker M., Garcia‑Alai. M., Knauer S.H., Lopez‑Mendez B., Matagne A., Parret A., Remans K., Uebel S., Raynal B. (2021)
de Marco A , Berrow N. , Lebendiker M , Garcia-Alai M, Knauer S. , Lopez-Mendez B. , Matagne A. , Parret A. , Remans K., Uebel S., Raynal B. (2021)

A team of experts in the field of Biophysics (Association of Resources for Biophysical Research in Europe-ARBRE-MOBIEU) and Recombinant Protein Production (Production and Purification Partnership in Europe-P4EU) have developed the following Minimal Protein Quality Standard:

Members of the Protein Quality Initiative:
Nick Berrow – Institute for Research in Biomedicine, Barcelona, Spain (P4EU)
Maria Garcia-Alai – EMBL Hamburg, Germany (ARBRE)
Stefan Knauer – Bayreuth University, Germany (ARBRE)
Mario Lebendiker – Hebrew University of Jerusalem, Israel (P4EU)
Blanca Lopez-Mendez – Novo Nordisk Foundation, Denmark (ARBRE)
Ario de Marco – University of Nova Gorica, Slovenia (P4EU)
André Matagne – University of Liège, Belgium (ARBRE)
Annabel Parret – Charles River Laboratories, Beerse, Belgium
Bertrand Raynal  – Pasteur institute, Paris, France (ARBRE)
Kim Remans – EMBL Heidelberg, Germany (P4EU)
Stephan Uebel  – Max-Plank Institute for Biochemistry, Munich (ARBRE)

shadow
The opinions and assertions of the PQS working group are supported by many other researchers in the field. Click to view.

Addendum-Updated-08062021

Minimal information to provide in publications. Click to view.

Minimal-information-to-provide-in-publications-1

Minimal quality control parameters that should be tested on protein sample. Click to view.

Minimal-quality-control-parameters-that-should-be-tested-on-protein-sample

Extended quality control parameters. Click to view.

Extended-Quality-Control-Parameters-2

The full guidelines are also available as a single pdf document here (downloads document).

There is also a 30-minute webinar presentation available recorded by André Matagne from the joint PQC working group as part of the FHI-Netherlands ‘Life Science Online 2021’ webinar series. The webinar can be viewed here- André Matagne-Quality control of purified proteins to improve research data reproducibility: improving the time-efficiency and quality of your results.

The proposed guidelines are intended to lay the groundwork for the standardization and reproducibility of data. The goals of this document are to disseminate operative guidelines in our laboratories through the existing networks, to raise awareness amongst colleagues and collaborators and to encourage the whole scientific community to implement these guidelines in publications, e.g. as part of the supplementary information. The implementation of protein production and QC data will allow greater transparency to readers and enable efficient reproducibility in other laboratories.

Projects and Benchmarks

PROSS Benchmarking
Insect and HEK benchmarking
Sf21 genome assembly

Courses

Protein quality control for downstream processes-Course, EMBL Heidelberg, 29 Nov – 2 Dec 2022
EMBO Practical Course in High throughput protein production and crystallization 4th to 13th July 2022, Marseille, France.
MOSBRI QC4Bio (Biophysical Characterization/ Protein Quality) Course, Institut Pasteur, 4th-8th of April 2022

Upcoming P4EU Meetings

The 18th P4EU Meeting and ‘Protein Engineering Tools’ workshop-Centre for Genomic Regulation (CRG), Barcelona, 30th May-1st June 2022.

Upcoming Conferences

New dates for the 1st MOSBRI Scientific Conference, Institut Pasteur, Paris. 20th-22nd June 2022.

Public Forum

Place to leave your comments to your work or discuss important topics.

Contact

Place to leave your comments to your work or discuss important topics.

Wiki

You can find useful information about the Protein technologies here and share your knowledge.

© Copyright 2022. All Rights Reserved | Powered by p4eu.org | Designed by AWA
  • Imprint
  • /
  • privacy policy
  • /
  • Cookie Policy (EU)
  • /
Manage Cookie Consent
We use cookies to optimize our website and our service.
Functional Always active
The technical storage or access is strictly necessary for the legitimate purpose of enabling the use of a specific service explicitly requested by the subscriber or user, or for the sole purpose of carrying out the transmission of a communication over an electronic communications network.
Preferences
The technical storage or access is necessary for the legitimate purpose of storing preferences that are not requested by the subscriber or user.
Statistics
The technical storage or access that is used exclusively for statistical purposes. The technical storage or access that is used exclusively for anonymous statistical purposes. Without a subpoena, voluntary compliance on the part of your Internet Service Provider, or additional records from a third party, information stored or retrieved for this purpose alone cannot usually be used to identify you.
Marketing
The technical storage or access is required to create user profiles to send advertising, or to track the user on a website or across several websites for similar marketing purposes.
Manage options Manage services Manage vendors Read more about these purposes
Preferences
{title} {title} {title}