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Temporary position (18 months) at the Mammalian Protein Expression core facility, University of Gothenburg, Sweden.

Temporary position (18 months) at the Mammalian Protein Expression core facility, University of Gothenburg, Sweden.

Duties 

The work is lab-based and involves culture and transfection of mammalian cells, protein purification, characterization and quality control. This includes planning and performance of different types of mammalian cell cultures, including large- and small-scale transfections and bioreactor cultures, purification of produced proteins and various protein analyses. Some molecular biology work and cloning of cells may occasionally be involved in the tasks.

The communication with different researchers and the reporting of projects are important parts of the work, as well as participating in maintaining the center’s routines. The work is carried out independently or together with other group members and requires flexibility and ability to adapt to the different tasks. Some weekend work may occur. The position requires professional knowledge and experience in protein production as well as a good understanding of both protein chemistry and the academic research environment. It is also important to be able to carry out experiments based on the researchers’ scientific demands in a time-efficient and quality-conscious manner.

 
Qualifications 

The successful applicant has a PhD in biochemistry, molecular biology or equivalent, with an interest in and long experience of work in protein chemistry, cell biology, molecular biology or other relevant biomedical field. Experience from the expression of recombinant proteins, protein purification using various chromatography-based methods and different techniques for the quality control of proteins are required. Experience of culturing mammalian cells for this purpose is highly meriting, as is documented experience of sterile work. You should also have experience of common molecular biology techniques and preferably also of working with various commonly used expression vectors and expression systems for mammalian cells. Knowledge and experience of working with protein glycosylation is desirable. Previous experience of large-scale cultivation of mammalian cells in bioreactors is a merit, as is the production of protein samples for structural analysis using Cryo-EM or other techniques. Previous work within a protein production research infrastructure is desirable.

Employment 

The employment is a substitute during an ongoing leave of absence between 2023-03-01 and 2024-08-31. The employment is full-time (100%) and is placed at Mammalian Protein [removed]MPE) Core Facility, Sahlgrenska Academy. Starting date is 2023-03-01 or as soon as agreed.

Application 

For further details and how to apply please visit:
https://web103.reachmee.com/ext/I005/1035/job?site=7&lang=UK&validator=9b89bead79bb7258ad55c8d75228e5b7&job_id=28693 

Applications must be received by: 2023-01-31

Projects and Benchmarks

PROSS Benchmarking
Insect and HEK benchmarking
Sf21 genome assembly

Courses

Protein quality control for downstream processes-Course, EMBL Heidelberg, 29 Nov – 2 Dec 2022
EMBOPractical Course Membrane Protein Expression, Purification and Characterization (mPEPC2). Hamburg, Germany. 5th-12th September 2022.

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19th P4EU meeting, Elettra Synchrotron and ICGEB -Trieste, 29th-30th May 2023. First announcement.

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