P4EU P4EU P4EU
Navigation
  • Home
  • About Us
    • Executive Board Members
    • Network Members
    • Members Publications
  • News
    • Projects & Benchmarks
    • Courses
    • Upcoming P4EU Meetings
    • Upcoming Conferences
  • Join Us
  • Protein Quality Standard PQS
  • Jobs
  • Contact
  • Archives
    • Courses
    • P4EU Meetings

Login:

Lost Password?

Shortcuts

  • Public Forum
  • Wiki
  • Contact
  • This topic has 2 replies, 3 voices, and was last updated 2 months, 2 weeks ago by Hüseyin Besir.
Viewing 2 reply threads
  • Author
    Posts
    • March 9, 2022 at 21:04 #4253

      Nikolay Dobrev
      Participant

      Dear all,
      I am looking for someone who can share any experience removing Ferritin contamination when purification from mammalian suspension cells is performed.
      The Ferritin contamination co purifies via gradient for AAV particles. If anyone knows some tricks how to convert the 24-mer Ferritin to a monomeric form ( besides the case with low pH) will be pretty helpful and appreciated.
      Furthermore, if someone is aware of specific affinity resin ( like nanobody) will be great to share.

      Thank you,
      Nikolay

    • March 11, 2022 at 10:03 #4254

      Mario Lebendiker
      Participant

      Hi Nikolay,
      the pI of Ferritin is near 5.5. So, around pH 7.5 8.0 it will possible bind to anion exchange columns more stronger than AAV. You can contact me if you need some advice how to do it.
      I would give this a chance
      Mario

    • April 12, 2022 at 11:37 #4279

      Hüseyin Besir
      Participant

      Hi Nikolay,
      if you haven’t tried Mario’s suggestion, here is a paper describing the separation of ferritin from an AAV preparation using ion exchange. The ferritin seems to bind tightly under these conditions.
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4817810/

      Hope that helps.
      Hüseyin

  • Author
    Posts
Viewing 2 reply threads

You must be logged in to reply to this topic.

Projects and Benchmarks

PROSS Benchmarking
Insect and HEK benchmarking
Sf21 genome assembly

Courses

EMBO Practical Course in High throughput protein production and crystallization 4th to 13th July 2022, Marseille, France.
MOSBRI QC4Bio (Biophysical Characterization/ Protein Quality) Course, Institut Pasteur, 4th-8th of April 2022
EMBOPractical Course Membrane Protein Expression, Purification and Characterization (mPEPC2). Hamburg, Germany. 5th-12th September 2022.

Upcoming P4EU Meetings

The 18th P4EU Meeting and ‘Protein Engineering Tools’ workshop-Centre for Genomic Regulation (CRG), Barcelona, 30th May-1st June 2022.

Upcoming Conferences

New dates for the 1st MOSBRI Scientific Conference, Institut Pasteur, Paris. 20th-22nd June 2022.

Public Forum

Place to leave your comments to your work or discuss important topics.

Contact

Place to leave your comments to your work or discuss important topics.

Wiki

You can find useful information about the Protein technologies here and share your knowledge.

© Copyright 2022. All Rights Reserved | Powered by p4eu.org | Designed by AWA
  • Imprint
  • /
  • privacy policy
  • /
  • Cookie Policy (EU)
  • /
Manage Cookie Consent
We use cookies to optimize our website and our service.
Functional Always active
The technical storage or access is strictly necessary for the legitimate purpose of enabling the use of a specific service explicitly requested by the subscriber or user, or for the sole purpose of carrying out the transmission of a communication over an electronic communications network.
Preferences
The technical storage or access is necessary for the legitimate purpose of storing preferences that are not requested by the subscriber or user.
Statistics
The technical storage or access that is used exclusively for statistical purposes. The technical storage or access that is used exclusively for anonymous statistical purposes. Without a subpoena, voluntary compliance on the part of your Internet Service Provider, or additional records from a third party, information stored or retrieved for this purpose alone cannot usually be used to identify you.
Marketing
The technical storage or access is required to create user profiles to send advertising, or to track the user on a website or across several websites for similar marketing purposes.
Manage options Manage services Manage vendors Read more about these purposes
Preferences
{title} {title} {title}