- This topic has 0 replies, 1 voice, and was last updated 1 year, 2 months ago by Mario Lebendiker.
April 8, 2019 at 15:39 #2262
I introduce a video film of how to run IEX-MALS.
Amartely, H., Some, D., Tsadok, A., Lebendiker, M.
Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization.
J. Vis. Exp. (146), e59408, doi:10.3791/59408 (2019).
For some applications I am using it directly during production as process monitoring during last step purification in IEX. Today we succeed to separate monomer from dimer of Synuclein and from other impurities. Immediately after run, you can get the exact mass of each peak in the ASTRA.
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