[Luigi Angelo Scietti]

Hi good people,

yesterday a user pointed me to this Expi293 PRO kit (Thermo), that also include Epxi293 PRO cells.
https://tinyurl.com/expi293pro

Apparently, yields are much higher than standard Expi293, at least for transient transfection.

Someone who tried them or know something more?
Is this higher yield due to cells or to transfection reagent?

Thanks in advance
have a nice day
Luigi

• This topic was modified 2 weeks, 1 day ago by Luigi Angelo Scietti.

[Laure Bataille]

Hi Luigi,

I was wondering exactly the same thing!

For now we are working with standard Expi293.. and i’ll have a meeting with thermo on monday to discuss about this new kit..

If someone has already tried it, I would also be interested on a feedback

Have a nice day

Laure

[James Errey]

yes we have been using them for a while now, the media is more expensive but we have seen improved yields for some proteins (not all) for membrane proteins its been double for GPCRs (on average) but ion channels we saw little improvement (for the proteins we tested). For biologics we did see an improvement the most was around 7 fold but it was case by case…. on average they are better but its how the cost of the kits/media aligns with the improved yield

[Erwan Boedec]

Hi,

I tested this cell line for the production of recombinant antibodies and observed a three- to seven-fold increase in protein yield.

[Svend Kjaer]

We have just tested the PRO kit with antibodies and seen a substantial increase as well (1 g/L).

So far, quite convinced about their performance

[Sabine Suppmann]

Dear Colleagues

please note, that you have started this conversation in the public forum. Generally such questions would be better placed in the confidential forum. This network should not be a platform for advertising products neither the opposite.
Exchanging experience with commercial products shpuld be treated confidentially.

Best
Sabine

[Luigi Angelo Scietti]

Hi Sabine,

sorry, did’t realise it was the public forum.

I’ll open another discussion in the confidential one so that you can cancel this in case.

sorry again,
Best
Luigi

[Sabine Suppmann]

Dear Colleagues

please note, that you have started this conversation in the public forum. Generally such questions would be better placed in the confidential forum. This network should not be a platform for advertising products neither the opposite.
Exchanging experience with commercial products should be treated confidentially.

Best
Sabine

• This reply was modified 2 weeks, 1 day ago by Sabine Suppmann.

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