[Veronika Klapsťova]

Dear all,

are there any reliable tools for predicting protein solubility from sequence, and possibly also for predicting disulfide bonds (although these can often be guessed from structure predictions)? Which tools have you tried and found most accurate (those that correctly predicted a protein to be soluble and it indeed was during expression)?

Have a nice day,
Veronika

• This topic was modified 2 days, 1 hour ago by Veronika Klapsťova.

[Chris Cooper]

Hi Veronika,

There’s CamSol for solubility analysis from the Vendruscolo Lab at Cambridge, but I think you need to register and not sure if you need a licence – lots of other resources there too:

https://www-cohsoftware.ch.cam.ac.uk//

Thanks! Chris

[Evgeny Tankhilevich]

Dear Veronika,

I am the author of RP3Net (GitHub, preprint), an AI model for predicting soluble expression in E. coli based on construct sequence. RP3Net is free to use; a few installation options are described on the GitHub page, and it can also be used without installation via Google Colab.

The preprint also references other solubility/expressability predictors: NetSolP and SoluProt.

Hope this helps.
Evgeny

[Author]

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