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    • January 17, 2018 at 17:39 #1173

      Tamar Unger
      Participant

      Are there any examples of recombinant proteins which are expressed intracellular in eukaryotic systems (yeast, insect or mammalian cells) that could be identified as a major band from cell extracts, without any manipulation, when analyzing on SDS-PAGE staining? (Not by immunoblot analysis and without partial purification).

    • January 17, 2018 at 18:23 #1174

      Hüseyin Besir
      Participant

      Hi Tamar,

      we had many intracellular insect cell expression constructs that gave a strong band in the total lysate on SDS-PAGE with the correct size of the target. Do you need any pictures or constructs? How about the constructs from the insect cell benchmarking experiments? Those were clearly visible in the crude lysate in most cases.
      Best regards

      Hüseyin

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