Viewing 1 reply thread
  • Author
    Posts
    • August 29, 2019 at 11:32 #2472

      Jan Blaha
      Participant

      Dear P4EU members,

      I was wandering if anyone adapted and optimized the TetON inducible piggyBac system (Rini et al. 2013) for generation of stable cell lines in HighFive cells? Do you have any experience with sensitivity of HighFive to doxycycline?

      Thanks

    • August 29, 2019 at 12:03 #2473

      David Hacker
      Participant

      Hello Jan,

      As HighFive cells are from a lepidopteran species the genome should already contain functional piggybac transposons, making these cells incompatible with the use of an exogenous piggybac transposon. We have used the piggybac system for the generation of stable S2 cells. However, we have only used a constitutive promoter. I can provide further information, if needed.

      Best regards,
      David Hacker
      Protein Production and Structure Core Facility
      EPFL
      1015 Lausanne, Switzerland

  • Author
    Posts
Viewing 1 reply thread

You must be logged in to reply to this topic.