- This topic has 1 reply, 2 voices, and was last updated 5 months ago by David Hacker.
August 29, 2019 at 11:32 #2472
Dear P4EU members,
I was wandering if anyone adapted and optimized the TetON inducible piggyBac system (Rini et al. 2013) for generation of stable cell lines in HighFive cells? Do you have any experience with sensitivity of HighFive to doxycycline?
August 29, 2019 at 12:03 #2473
As HighFive cells are from a lepidopteran species the genome should already contain functional piggybac transposons, making these cells incompatible with the use of an exogenous piggybac transposon. We have used the piggybac system for the generation of stable S2 cells. However, we have only used a constitutive promoter. I can provide further information, if needed.
Protein Production and Structure Core Facility
1015 Lausanne, Switzerland
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